In-source fragmentation

Positive ion mode

Lipid classes	In-source fragmentation*
Cer	NL of one or two molecules of water – generation of isomeric species like [Cer d34:1+H]+ = [Cer t34:0+H-H2O]+ and isobaric species [Cer d34:1+H]+ = [Cer t34:0+H-H2O]+
CE – FC	NL of FA – generation of cholestadien ion
SM – SPC	NL of FA – generation of SPC
HexCer – Cer	NL of hexose – generation of Cer
SHexCer – HexCer	NL of SO3 – generation of HexCer
Sterols	NL of water – generation of [M+H-H2O]+
DG and MG	NL of water from [M+Na]+ or [M+NH4]+
TG – DG	NL of FA – generation of DG
PS and PC – PA	NL of serine/choline headgroup – generation of PA
LPS and LPC – LPA	NL of serine/choline headgroup – generation of LPA
PL – LPL	NL of FA – generation of LPL

Negative ion mode

Lipid classes	In-source fragmentation*
PL and SL	Generation of [RCOO]–
PS – PA	NL of serine headgroup – generation of PA
LPS – LPA	NL of serine headgroup – generation of LPA