Separation MS

• This section describes guidelines for various separation techniques, i.e.:
  • liquid chromatography, LC
  • gas chromatography, GC
  • supercritical fluid chromatography, SFC
  • ion mobility (IM)
• Lipid species identification is based on principles discussed for for direct infusion MS
• Retention time should be applied as an additional criteria for lipid species identification in chromatography-MS methods

Lipid class separation

• LC, SFC: polar stationary phases – NP, HILIC
• Retention times should match lipid class
• Separation of isomeric lipid classes or isomeric lipid species need to be demonstrated by authentic standards

Lipid species separation

• LC, SFC: non-polar stationary phases – RP
• Retention times should match the structural features of the lipid species, i.e. content of DB, alkyl/acyl-bond
• Separation of isomeric lipid species need to be demonstrated by authentic standards or substantiated by retention time models