Preanalytics

Preanalytics comprise collection, preparation and storage of the samples.

• The following should be shown together with the data:
  • Type of sample
  • Sample collection details, e.g. amount, collection tubes, cell passages
  • Sample preparation details
  • Storage conditions
  • Freeze-thaw cycles
• Current best practice:
  • Collect, prepare and store samples on ice
  • Immediately flash-freeze in liquid nitrogen
  • Long-time storage at -150°C – -80°C
• Evaluation of preanalytic requirements for the sample material is highly recommended e.g.:
  • Stability at different temperatures
  • Stability during preparation and storage e.g. impact of storage containers and solvents
  • For blood samples – time to separate plasma/serum
  • For lipid mediators – prevention of artifactual generation e.g. addition of organic solvents, use of additives or enzyme inhibitors.