report – lipidomicstandards.org

Title of the study *

Principle investigator *

Please specify the person / laboratory responsible for the data acquisition.

Institution *

Please specify the institution where the data was acquired.

Corresponding Email *

To whom correspondence should be addressed?

Is the workflow targeted or untargeted? *

Clinical *

Is it clinical lipidomics, i.e. samples were analyzed related to a clinical question using a quantitative, validated method?

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Sample types

Registered sample types to workflows

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Extraction method *

Which type of lipid extraction was applied?

Other extraction method *

Which other extraction method was applied?

pH adjustment *

Was the pH adjusted for extraction? If yes, which buffer, acid/base was added?

1-phase system *

Type of 1-phase, i.e. organic solvent used for protein precipitation.

Other 1-phase system *

Which other type 1-phase was used for protein precipitation?

2-phase system *

Type of 2-phase; for other include reference when available.

Other 2-phase system *

Which other type 2-phase was used?

Solid-Phase Extraction *

Separation mode applied for SPE.

Other Solid-Phase Extraction *

Which other solid-phase extraction was applied?

Were internal standards added prior extraction? *

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Number of separation dimensions

How many dimensions were applied?

Separation Type 1

Which type of separation was applied?

Separation Mode 1

Which separation mode was applied?

Separation window (1) for lipid analyte selection (±) in minutes *

Separation Type 2

Which type of separation was applied?

Separation Mode 2

Which separation mode was applied?

Separation window (2) for lipid analyte selection (±) in minutes *

Separation Type 3

Which type of separation was applied?

Separation Mode 3

Which separation mode was applied?

Separation window (3) for lipid analyte selection (±) in minutes *

RT verified by standard

Were standards used to check retention time of lipid class(species)?

CCS verified by standard

Were standards used to check CCS values?

Separation of isobaric/isomeric interferece confirmed

Was separation of isobaric/isomeric interferece confirmed by primary standards?

Model for separation prediction

Was a a model used to predict lipid molecule separation?

MS type

Other MS type *

Specify another MS type.

MS vendor

Vendor of mass spectrometer.

Other MS vendor *

Specify another MS vendor.

Ion source

Which ion source was applied?

Other ion source *

Specify another ion source.

Imaging ion source

Which ion source was applied?

Other imaging ion source *

Specify another imaging ion source.

Direct type

Which inlet was used for direct MS?

MS Level *

Which MS level(s) was(were) used for identification?

Mass resolution for detected ion at MS1

Which mass resolution was applied for the detected ion?

Resolution in Da at MS1 *

Specify the total window size in Da.

Resolution at m/z 200 at MS1 *

Specify the resolution at m/z 200 FWHM.

Mass accuracy in ppm at MS1 *

Mass window for precursor ion isolation (in Da total isolation window) *

For MS2 and higher - which mass window was applied for precursor selection?

Mass resolution for detected ion at MS2

Which mass resolution was applied for the detected ion?

Resolution in Da at MS2 *

Specify the total window size in Da.

Resolution at m/z 200 at MS2 *

Specify the resolution at m/z 200 FWHM.

Mass accuracy in ppm at MS2 *

Mass resolution for detected ion at MSn

Which mass resolution was applied for the detected ion?

Resolution in Da at MSn *

Specify the total window size in Da.

Resolution at m/z 200 at MSn *

Specify the resolution at m/z 200 FWHM.

Mass accuracy in ppm at MSn *

Additional dimension/techniques

IMS
OzID
UV-PD
Paterno-Büchi
Other

Were additional analytical dimensions applied?

Other additional dimension/techniques *

Which other additional dimension/technique was used?

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Lipid Classes

Registered Lipid classes to workflows

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Blanks *

Where blank samples analyzed?

Type of Blanks *

Extraction blank
Injection blank

Which types of blank samples were analyzed?

Quality control *

Were QC samples analyzed?

Type of QC sample *

Commercial sample
Sample pool
Reference material

Which types of QC samples were analyzed?

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Method validation *

Was any method validation performed?

Lipid recovery

Was the recovery of lipid extraction evaluated?

Dynamic quantification range

Was the dynamic range of the method evaluated?

Limit of quantitation (LOQ)/Limit of detection (LOD)

Was LOD/LOQ of the method evaluated?

Precison

Was precision of the method evaluated?

Accuracy

Was accuracy of the method evaluated?

Guidelines followed

Was a guideline followed for method validation?

Other guidelines followed *

Which other guidelines were followed?

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Are reported raw data uploaded into repository? *

Were reported data uploaded into repository or included as supplementary data?

Are metadata available?

Summary data

Quantification data
Identification data
Quantification and identification data

Are summary, i.e. processed final data available?

Raw data upload

Are raw data, i.e. unprocessed data including peak m/z, intensities/areas, RT (when applicable) available?